Human Pyruvate carboxylase, mitochondrial (PC) ELISA Kit

This Human Pyruvate carboxylase, mitochondrial (PC) ELISA Kit employs a two-site sandwich ELISA to quantitate PC in samples. An antibody specific for PC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PC is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PC bound in the initial step. The color development is stopped and the intensity of the color is measured.

Cell culture supernatants#Serum#Plasma#Other biological fluids

Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.

Human Pyruvate carboxylase, mitochondrial (PC) ELISA Kit has high sensitivity and excellent specificity for detection of Human PC. No significant cross-reactivity or interference between Human PC and analogues was observed.

The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

5091

P11498