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Human Protein S100-A16 (S100A16) ELISA Kit

Hersteller Abbkine Scientific
Kategorie
Typ Elisa-Kit
Specific against Human
Applikationen ELISA
Menge 48T
ArtNr ABK-KTE60767-48T
Targets S100A16;;S100B
eClass 6.1 32160605
eClass 9.0 32160605
Lieferbar
Applications notes
This Human Protein S100-A16 (S100A16) ELISA Kit employs a two-site sandwich ELISA to quantitate S100A16 in samples. An antibody specific for S100A16 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyS100A16 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A16 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A16 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Human Protein S100-A16 microplate
Human Protein S100-A16 standard
Human Protein S100-A16 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Human Protein S100-A16 (S100A16) ELISA Kit has high sensitivity and excellent specificity for detection of Human S100A16. No significant cross-reactivity or interference between Human S100A16 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
Protein S100-A16 is a protein encoded by the S100A16 gene.S-100 protein is a family of low molecular weight protein found in vertebrates characterized by two calcium binding sites of the helix-loop-helix ("EF-hand type") conformation. There are at least 21 different types of S100 proteins. The name is derived from the fact that the protein is 100% Soluble in ammonium sulfate at neutral pH.Most S100 proteins are homodimeric, consisting of two identical polypeptides held together by non-covalent bonds. Although S100 proteins are structurally similar to calmodulin, they differ in that they are cell-specific, expressed in particular cells at different levels depending on environmental factors. To contrast, calmodulin is a ubiquitous and universal intracellular Ca++ receptor widely expressed in many cells.
Gene ID
140576
Alternative
S100A16; AAG13; DT1P1A7; MGC17528; S100F; OTTHUMP00000015465; OTTHUMP00000015466; OTTHUMP00000032894; aging-associated protein 13
Accession
Q96FQ6

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 48T
Lieferbar: In stock
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