Human Transient receptor potential cation channel subfamily M member 1 (TRPM1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Human Transient receptor potential cation channel subfamily M member 1 microplate
Human Transient receptor potential cation channel subfamily M member 1 standard
Human Transient receptor potential cation channel subfamily M member 1 detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Transient receptor potential cation channel subfamily M member 1 is a protein similar to the transient receptor potential (Trp) calcium channel family members. The expression of this protein is inversely correlated with melanoma aggressiveness, suggesting that it suppresses melanoma metastasis. The expression of the TRPM1 gene is regulated by the Microphthalmia-associated transcription factor. Northern blot analysis of mouse tissues and cell lines revealed that melastatin was expressed as a 2.8-kb mRNA in normal eye and in 4 melanoma cell lines; its expression in each of the 4 cell lines was inversely proportional to metastatic potential. In 45 human melanocytic primary neoplasms examined by in situ hybridization, the loss of melastatin expression correlated with the thickness of the melanomas.

TRPM1; LTRPC1; MLSN1; melastatin 1