Vergleich

Chicken Myosin-VI (MYO6) ELISA Kit

ArtNr ABK-KTE30084-96Tx50
Hersteller Abbkine Scientific
Menge 96Tx50
Kategorie
Typ Elisa-Kit
Applikationen ELISA
Specific against Gallus
ECLASS 10.1 32160605
ECLASS 11.0 32160605
UNSPSC 41116126
Lieferbar
Applications notes
This Chicken Myosin-VI (MYO6) ELISA Kit employs a two-site sandwich ELISA to quantitate MYO6 in samples. An antibody specific for MYO6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyMYO6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for MYO6 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of MYO6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Detection method
Colorimetric
Sample type
Cell culture supernatants#Serum#Plasma#Other biological fluids
Assay type
Sandwich ELISA (quantitative)
Assay duration
Multiple steps standard sandwich ELISA assay with a working time of 3-5 hours. It depends on the experience of the operation person.
Kit components

Chicken Myosin-VI microplate
Chicken Myosin-VI standard
Chicken Myosin-VI detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers
Features & Benefits
Chicken Myosin-VI (MYO6) ELISA Kit has high sensitivity and excellent specificity for detection of Chicken MYO6. No significant cross-reactivity or interference between Chicken MYO6 and analogues was observed.
Usage notes
Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.
Storage instructions
The unopened kit should be stored at 2 - 8C. After opening, please store refer to protocols.
Shipping
Gel pack with blue ice.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Background
MYO6 encodes a reverse-direction motor protein that moves toward the minus end of actin filaments and plays a role in intracellular vesicle and organelle transport. Myosin-VI consists of a motor domain containing an ATP- and an actin-binding site and a globular tail which interacts with other proteins. Myosin-VI maintains the structural integrity of inner ear hair cells and mutations in this gene cause non-syndromic autosomal dominant and recessive hearing loss. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
Gene ID
395487
Alternative
MYO6; RP3-472A9.1; DFNA22; DFNB37; KIAA0389;
Accession
Q9I8D1

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Alle Produkte sind nur für Forschungszwecke bestimmt. Nicht für den menschlichen, tierärztlichen oder therapeutischen Gebrauch.

Menge: 96Tx50
Lieferbar: In stock
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