Bovine Lanosterol 14-alpha demethylase (CYP51A1) ELISA Kit

Colorimetric

Sandwich ELISA (quantitative)

Bovine Lanosterol 14-alpha demethylase microplate
Bovine Lanosterol 14-alpha demethylase standard
Bovine Lanosterol 14-alpha demethylase detect antibody
Streptavidin-HRP
Standard diluent
Assay buffer
HRP substrate
Stop solution
Wash buffer
Plate covers

Do not mix components from different kit lots or use reagents beyond the kit expiration date.
Allow all reagents to warm to room temperature for at least 30 minutes before opening.
Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
Unused wells must be kept desiccated at 4 C in the sealed bag provided.
Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
It is recommended that all samples and standards be assayed in duplicate or triplicate.

Gel pack with blue ice.

Lanosterol 14-alpha demethylase (CYP51A1) has a alternative name : Cytochrome P450 Family 51 Subfamily A Member 1, This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This endoplasmic reticulum protein participates in the synthesis of cholesterol by catalyzing the removal of the 14alpha-methyl group from lanosterol. Homologous genes are found in all three eukaryotic phyla, fungi, plants, and animals, suggesting that this is one of the oldest cytochrome P450 genes. This protein acts as an enzyme. It is known to catalyze the following reaction : A 14-alpha-methylsteroid + 3 O(2) + 3 NADPH = a Delta(14)-steroid + formate + 3 NADP(+) + 4 H(2)O.

CYP51A1; CP51; CYP51; CYPL1; LDM; P450-14DM; P450L1; cytochrome P450; 51 (lanosterol 14-alpha-demethylase); lanosterol 14-alpha-demethylase; sterol 14-alpha demethylase