BL21 (DE3) Chemically Competent Cell is designed for chemical transformation of DNA, with a transformation efficiency of over 10
7 cfu/µg DNA (tested by pUC19 plasmid DNA). The DE3 strain contains the λ DE3 lysogen, which carries the gene for T7 RNA polymerase. The control plasmid (Amp
+) is used for detection of cell expression functions (protein size: ~25 kDa). Suitable for high expression of non-toxic proteins.
The genotype is: F
- ompT
hsdS(r
B-m
B-)
gal dcm(DE3).
The 1 ml size consists of 10 × 100 µl Competent Cells, and 20 μl (0.1 ng/μl) Control Plasmid pUC19.