BCA Protein Assay Kit (Enhanced)

The BCA protein assay is a protein determination formulation based on bicinchoninic acid (BCA) for the colorimetric detection. This method combines the reduction of Cu2+ to Cu+ by proteins in an alkaline medium (the biuret reaction) and the soluble purple-colored reaction product from the complexing of Cu+ and BCA. This purple-colored complex exhibits a maximum absorbance at 562 nm that is nearly linear with increasing protein concentrations over a broad working range (0-2 mg/ml). Since it is highly sensitive and simple to use, the BCA protein assay is adopted by many laboratories and companies, and is one of the main protein quantitative methods as well as the Bradford assay.

Not applicable.