RIPA Lysis Buffer (Strong)

Radioimmunoprecipitation Assay (RIPA) buffer is a lysis buffer used to lyse cells and tissue, for Western Blot and Immunoprecipitation. This buffer is more denaturing than NP-40 or Triton X-100 lysis buffer because it contains the ionic detergents SDS and sodium deoxycholate as active constituents, and is particularly useful for disruption of nuclear membranes in the preparation of nuclear extracts.

The concentration of the protein in the lysed pellet can be determined by our BCA protein assay kits (abx090640 and abx090642). It is not recommended to use a Bradford Protein Assay Kit with this buffer.

Contains 50 mM Tris (pH 7.4), 150 mM NaCl, 1% Triton X-100, 1% sodium deoxycholate, 0.1% SDS, and inhibitors (sodium orthovanadate, sodium fluoride, EDTA and leupeptin).

This product is for research use only.