DB3.1 Chemically Competent Cell is designed for chemical transformation of DNA. This cell contains the
gyrA462 gene which provides resistance to the toxic effects from the
ccdB gene. This product can be used for transformation and propagation of plasmids containing the
ccdB gene, with a transformation efficiency of over 10
8 cfu/µg DNA (tested by pUC19 plasmid DNA).
The genotype is: F
- gyrA462
endA1 Δ (
sr1-
recA)
mcrB
mrr hsdS20(r
B-, m
B-)
supE44
ara-14
galK2
lacY1
proA2
rpsL20(Sm
R)
xyl-5 λ -
leu mtl1.
The 1 ml size consists of 10 × 100 µl Competent Cells, 20 μl (0.1 ng/μl) Control Plasmid pUC19, and 10 ml SOC Medium.